Amino acid sequences C-terminal to the cross-links in bovine elastin

The N-terminal amino-acid sequence of bovine proparathyroid hormone.

Proparathyroid hormone (calcemic fraction-A) is a biosynthetic precursor of parathyroid hormone in bovine glands. Limited amounts of the isolated prohormone have been obtained for the purpose of initial structural studies. The results of automated sequence analysis on two separate preparations indicate that the N-terminal region of the prohormone consists of the sequence Lys-Ser-Val-Lys-Lys-Arg...

Spinach ferredoxin. I. Amino acid composition and terminal sequences.

The amino acid composition of several preparations of spinach ferredoxin was determined, and the most reliable composition was concluded to be: Lysd, Hisr, Argr, Trpr, Asp,,, Thr8, Ser?, Glu13, Prod, GUYS, Alas, CYSS, V&, Meto, Ile4, Leus, Tgr4, Phez, (NH3)6. The total number of amino acid residues is 9’7. The molecular weight of spinach ferredoxin was determined by gel tiltration, amino acid c...

The amino acid sequence of bovine heart cytochrome c.

Bovine protein C: amino acid sequence of the light chain.

The amino acid sequence of the light chain of bovine protein C was determined by sequenator analysis of the carboxymethylated light chain and fragments obtained by cyanogen bromide treatment, tryptic digestion after blocking of lysine residues, and cleavage with 2-(2-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine (BNPS-skatole). The sequence was (in the standard one-letter code) A-N-S-F-L-X-X-...

Ultraviolet radiation reduces desmosine cross-links in elastin

Elastic fibers, a major component of the extracellular matrix of the skin, are often exposed to ultraviolet (UV) radiation throughout mammalian life. We report on an in vitro study of the alterations in bovine nuchal ligament elastic fibers resulting from continuous UV-A exposure by the use of transmission electron microscopy (TEM), histology, mass spectrometry, and solid state 13C NMR methodol...